Fig. 5: Tim-3+dMφs and Tim-3−dMφs showed distinct CD132 expression indicative of different functional regulation on dCD4+T cells. | Cell Death & Disease

Fig. 5: Tim-3+dMφs and Tim-3−dMφs showed distinct CD132 expression indicative of different functional regulation on dCD4+T cells.

From: Tim-3+ decidual Mφs induced Th2 and Treg bias in decidual CD4+T cells and promoted pregnancy maintenance via CD132

Fig. 5

A The volcano plot and cluster analysis heat map of the differential mRNAs between Tim-3+dMφs and Tim-3−dMφs form human normal early pregnancy. Volcano plot based on fold change and p value of genes from the unique signatures of Tim-3+dMφs and Tim-3−dMφs in comparison with each other. Red dots mark the genes with significantly increased or decreased expression. Heatmap result of an unsupervised hierarchical clustering of genes that is significantly different in Tim-3+dMφs samples compared with Tim-3−dMφs samples. For example, gene encoding IL2RG was overexpressed in the Tim-3+dMφs population as compared with the Tim-3−dMφs. B Flow cytometric analysis and quantification of CD132 (the protein product of IL2RG gene) by human Tim-3+dMφs and Tim-3−dMφs (n = 9). **p < 0.01, compared with group of Tim-3+dMφs. The flow cytometry plots are representative of three independent experiments. C Flow cytometric analysis and quantification of Th2- and Treg-related cytokine expression by human dCD4+ T cells co-cultured with Tim-3+dMφs in the present or absence of anti-Tim-3 antibody, anti-CD132 antibody, or anti-IL-4 antibody. Data represent the mean ±SEM. *p < 0.05, **p < 0.01, compared with the control group. #p < 0.5, ##p < 0.01, ###p < 0.001, compared with the group of Tim-3+dMφs.

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