Fig. 6: LIF is the direct target gene of both miR-140-3p and miR-335. | Cell Death & Disease

Fig. 6: LIF is the direct target gene of both miR-140-3p and miR-335.

From: CircSCAF8 promotes growth and metastasis of prostate cancer through the circSCAF8-miR-140-3p/miR-335-LIF pathway

Fig. 6

A Bioinformatic analysis predicted that LIF was the potential direct target gene of both miR-140-3p and miR-335. B, C qRT-PCR analysis showed that miRNA inhibitors increased the expression level of LIF, while miRNA mimics suppressed the expression level of LIF. D, F Western blot results showed the expression level of LIF after transfecting with miRNA inhibitors or miRNA mimics in PC3 cells. E, G Dual-luciferase reporter assays revealed that both miR-140-3p and miR-335 could bind to the 3′ UTR of LIF, and could suppress the luciferase ability. H Pearson correlation analyses showed that both miR-140-3p and miR-335 in PCa tissues (n = 30) were negatively correlated with LIF. Error bars represent the SD of three independent experiments. **P < 0.01; ***P < 0.001; ns nonsignificant.

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