Fig. 9: CircSCAF8 promotes growth and metastasis of PCa cells in vivo.

A BALB/c nude mice were anesthetized and sacrificed at the experimental endpoint, and subcutaneous xenograft tumors formed by PC3 cells were dissected and photographed (n = 8 for each group). B The subcutaneous tumor volumes were measured once a week and the growth curves were shown. C Each subcutaneous tumor at the experimental endpoint was weighed. D Orthotopic tumor growth of PC3 cells was monitored weekly by in vivo bioluminescence imaging. Two representative images are shown for each time point. (n = 7 for each group) E Orthotopic tumor bioluminescence signals were measured by mean photon radiance. F–I Representative images F, G, hematoxylin and eosin staining (I), and quantification (H) of lung metastatic colonization of NOD/SCID mice treated with a tail-vein injection of RM-1 cells (n = 7 for each group). J, K Representative images of IHC staining and quantitative analyses showing the intratumoral expression of LIF and p-STAT3 within orthotopic xenografts (n = 7 for each group). Error bars represent the SD. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. PCa prostate cancer, IHC immunohistochemistry.