Fig. 3: Deletion of Trp53 and Rb1 increases the expansion and differentiation ability of chondrocytes. | Cell Death & Disease

Fig. 3: Deletion of Trp53 and Rb1 increases the expansion and differentiation ability of chondrocytes.

From: Mice with Trp53 and Rb1 deficiency in chondrocytes spontaneously develop chondrosarcoma via overactivation of YAP signaling

Fig. 3

A Cell proliferation rate of primary chondrocytes and chondrosarcoma cells from Col2-Cre;Trp53f/f/Rb1f/f mice and age-matched controls (Col2-Cre) as shown by WST-1 assay. B, C Soft agar after culture of 3 weeks using primary chondrocytes and chondrosarcoma cells from Col2-Cre;Trp53f/f/Rb1f/f mice and age-matched controls, respectively (B). Cell numbers were quantified in the corresponding column as indicated (C). N = 3. D Migration and invasion as indicated. E Migration and invasion were quantified in the corresponding column as indicated. N = 3. F, G Tumorspheres were cultured as indicated for 3 days using primary chondrosarcoma cells from Col2-Cre;Trp53f/f/Rb1f/f mice and age-matched controls. The diameter of tumorspheres was quantified in the corresponding column as indicated (G). N = 3. H Micromass culture primary chondrosarcoma cells and chondrocytes from Col2-Cre;Trp53f/f/Rb1f/f mice and age-matched controls from Col2-Cre; Trp53f/f/Rb1f/f mice and controls. After a culture of 10 days, the macromass cultures as indicated were stained by Alcian blue staining. N = 3. Error bars were the means ± SEM from three independent experiments. *P < 0.05, **P < 0.01 ***P < 0.001.

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