Fig. 5: Metformin inhibits chondrosarcoma progression through YAP signaling. | Cell Death & Disease

Fig. 5: Metformin inhibits chondrosarcoma progression through YAP signaling.

From: Mice with Trp53 and Rb1 deficiency in chondrocytes spontaneously develop chondrosarcoma via overactivation of YAP signaling

Fig. 5

A Docking between YAP (6GE3 from PDB) and metformin. B After treatment of different doses of metformin as shown, the cell proliferation was determined at D0, D1, and D2 using primary chondrosarcoma cells from Col2-Cre;Trp53f/f/Rb1f/f mice. C Representative images of migration and invasion after treatment of different doses of metformin as indicated using primary chondrosarcoma cells. D The quantitative analysis of migration and invasion based on C. N = 3. E, F Representative images of soft agar (E) and quantitative analysis (F) after treatment of metformin as shown for 3 weeks. N = 3. G, H Representative images of tumorsphere (G) and quantitative analysis (H) after treatment of metformin for 3 days as indicated. N = 3. I Metformin inhibits YAP nuclear translocation. J After treatment with metformin of 48 h, luciferase activities were identified as indicated. K qRT-PCR analysis of YAP target genes’ expression. Error bars were the means ± SEM from three independent experiments. *P < 0.05, **P < 0.01.

Back to article page