Fig. 6: Loss of Kindlin-2 inhibits breast tumor metastasis in vivo. | Cell Death & Disease

Fig. 6: Loss of Kindlin-2 inhibits breast tumor metastasis in vivo.

From: Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression

Fig. 6

A Representative gross images of lung metastatic nodules from the PyMT;WT and PyMT; Kindlin-2 cKO littermates at 15 weeks of age. B Representative histological analyses of lung metastases by H&E staining. Original magnification: ×10. Quantification analysis of the number (C) and the areas (D) of lung metastatic nodules from PyMT;WT and PyMT; Kindlin-2 cKO mice at 15 weeks of age. ***p < 0.001 vs. PyMT;WT, n = 9. E BT549 cells were infected with lentiviral vectors encoding control shRNA (Ctrl shRNA) or Kindlin-2 shRNA (K2 shRNA). Five days after infection, the cells were harvested and analyzed by immunoblotting with antibodies as indicated. F Histological analyses of lung metastases from mice tail injection with Kindlin-2 knockdown (K2 shRNA) or control (Ctrl shRNA) BT549 cells. Original magnification: ×10 for the upper panels and ×100 for the lower panels. G Quantification analysis of the areas of lung metastatic nodules. ***p < 0.001 vs. Ctrl shRNA, n = 5.

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