Fig. 1: SKP2 is up-regulated both in ccRCC tissues and cells, and enhanced SKP2 can promote cell growth. | Cell Death & Disease

Fig. 1: SKP2 is up-regulated both in ccRCC tissues and cells, and enhanced SKP2 can promote cell growth.

From: Aurora-A/FOXO3A/SKP2 axis promotes tumor progression in clear cell renal cell carcinoma and dual-targeting Aurora-A/SKP2 shows synthetic lethality

Fig. 1

A Bar graph showing expression values of SKP2 gene using the study dataset, and data from http://ualcan.path.uab.edu. The 95% confidence interval was also displayed. B Protein expression of SKP2 in ccRCC and relationship with patient survival: Kaplan–Meier survival analysis indicated that patient with higher expression of SKP2 was related to a worse overall survival (log-rank test, p < 0.001). C Immunoblotting (IB) analysis of SKP2 and VHL expression in ccRCC tissues (T) and normal adjacent tissues (N), and ccRCC cell lines. D Depletion SKP2 observably suppressed cell proliferation and clonogenic ability in ccRCC cells. 786-O cells were transfected siRNAs targeting SKP2, and followed by IB, CCK-8 and colony formation assay, respectively. E Overexpression SKP2 significantly stimulated cell proliferation and clonogenic ability in ccRCC cells. A498 cells were transfected with vector or FLAG-SKP2, and followed by IB, CCK-8 and colony formation assay, respectively. The protein levels were normalized to GAPDH. ns no significance; *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 independent experiments; two-tailed paired or unpaired Student’s t test or one-way ANOVA with Dunnett’s test.

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