Fig. 7: TP53TG1 regulates MYH9 expression through binding to it.
From: Systematic analyses identify the anti-fibrotic role of lncRNA TP53TG1 in IPF
a The FISH probe detected that TP53TG1 was expressed in the cytosol and nucleus in MRC-5; bar = 20 μm; n = 4. b Schematic diagram of ChIRP-MS. c, d KEGG and GO analysis of the TP53TG1 interactome demonstrated the enrichment of splicing factors among TP53TG1-interacting proteins. e The interaction network of TP53TG1 protein interactome. f The protein mass spectrum suggested the MYH9 protein may bind to the TP53TG1. g RNA-binding protein immunoprecipitation (RIP) confirmed the interactions between TP53TG1 and MYH9 in vitro. h TP53TG1 derepressed the MYH9 protein in MRC-5. i In PMLFs, the expression of MYH9 was reduced by TP53TG1 overexpression. j, k The protein level of MYH9 in lung tissue of AAV-TP53TG1 mice was significantly inhibited than that of BLM-treated mice. *P < 0.05. AAV-TP53TG1, adeno-associated virus 5 containing TP53TG1; FC fold change.
