Fig. 1: Effects of dexamethasone on glucose oxidase-induced hair cell loss in vitro. | Cell Death & Disease

Fig. 1: Effects of dexamethasone on glucose oxidase-induced hair cell loss in vitro.

From: The protective effects of systemic dexamethasone on sensory epithelial damage and hearing loss in targeted Cx26-null mice

Fig. 1

A Diagram of cochlear explants culture, cochlear basilar membrane dissected from C57BL/6 mice at P3 and cultured in vitro. B, C The intracellular ROS level was measured with peroxide-sensitive fluorescent probe DCFH-DA in control group and GO group. D–O Immunofluorescence staining with myosin 7a (green) and DAPI (blue) in different turns of the cochleae from different group. Representative images of the control group, DEX, GO, and GO + DEX group are shown. The white arrowheads in panels J–L indicate the degeneration of hair cells. P Quantification of the survival rate of IHCs in different treatment groups. Q Quantification of the survival rate of OHCs in different treatment groups. Data are shown as the mean ± SD, n = 5 cochlear explants in each group, **P < 0.01, *P < 0.05. Scale in panels (B) and (D) represents for 40 μm.

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