Fig. 3: Effects of DEX or NAC on the degeneration of outer hair cell in specific longitudinally Cx26-null mice. | Cell Death & Disease

Fig. 3: Effects of DEX or NAC on the degeneration of outer hair cell in specific longitudinally Cx26-null mice.

From: The protective effects of systemic dexamethasone on sensory epithelial damage and hearing loss in targeted Cx26-null mice

Fig. 3

A Schematic of the experimental workflow, Cx26f/f; Lgr5CreER mice were injected with TMX and then treated with dexamethasone 5 mg /kg or NAC 300 mg /kg for five days. B, C Cx26 immunolabeling (red) in the apical of the control group. D, E Cx26 immunolabeling (red) in the apical of the specific longitudinally Cx26-null group. The asterisk indicates that Cx26 of the third row DCs was successfully knocked out. F–I Representative images of the flattened cochlear preparations in specific longitudinally Cx26-null group. J Magnifications of the yellow box in the panel (I), the white arrowheads in panel J indicated the OHC3 loss. K–N Representative images of the flattened cochlear preparations in Cx26-null + NAC group. O Magnifications of the yellow box in the panel (N). P–S Representative images of the flattened cochlear preparations in Cx26-null + DEX group. T Magnifications of the yellow box in the panel (S). U Quantifications of OHC3 loss in the different groups. Scales in panels (B) and (K) represent 40 and 200 μm, respectively.

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