Fig. 6: Effects of dexamethasone on the number and morphology of macrophage in DC-targeted Cx26-null mice. | Cell Death & Disease

Fig. 6: Effects of dexamethasone on the number and morphology of macrophage in DC-targeted Cx26-null mice.

From: The protective effects of systemic dexamethasone on sensory epithelial damage and hearing loss in targeted Cx26-null mice

Fig. 6

A Schematic of the experimental workflow, Cx26f/f; Fgfr3-CreER mice were treated with TMX and then treated with dexamethasone 5 mg /kg of body weight for five days. B–E Immunofluorescence staining with DAPI to show the pattern of hair cell damage from different group. F–I Immunofluorescence staining with CD45 (green) to visualized the distribution and morphology of macrophages in the basilar membrane from different groups. J–M The three-dimensional reconstruction of CD45 positive cells to show the morphology of CD45 positive cells from different groups. N Quantification of the numbers of CD45 positive cells on the scala tympani side of the basilar membrane in different groups. O Quantifications of CD45 positive cells size in three turns from different group. n = 4 mice in each group, **P < 0.01, ***P < 0.001. Scale in panels (B, C) represents for 40 μm.

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