Fig. 2: COL18A1-AS1 repressed ccRCC progression and eliminated lipids accumulation in vitro. | Cell Death & Disease

Fig. 2: COL18A1-AS1 repressed ccRCC progression and eliminated lipids accumulation in vitro.

From: Restoring the epigenetically silenced lncRNA COL18A1-AS1 represses ccRCC progression by lipid browning via miR-1286/KLF12 axis

Fig. 2

A qRT-PCR assays were applied to analyze the expression level of COL18A1-AS1 after transfection by si-RNA or COL18A1-AS1 overexpression vector for 24 h in RCC cells. B, C Cell proliferation ability of RCC cells after knocking down or overexpressing COL18A1-AS1 was determined using CCK8 assays or colony formation assays. D, E Cell migration and invasion ability of RCC cells was measured with Transwell assays or wound healing assays (Magnification: ×100 for Transwell assays and x40 for wound healing assays). F Photomicrographs of Oil Red staining of RCC cells knocked down or overexpressed COL18A1-AS1 compared with the negative control. Scale bars, 10 μm. G Relative TG (mmol/gprot) levels in RCC cells with COL18A1-AS1 overexpression or knockdown assessed by a triglyceride assay kit. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate mean ± SD. All the experiments were performed in triplicate.

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