Fig. 2: S100A11 facilitates cell proliferation, migration and stem cell potential of PDAC cells. | Cell Death & Disease

Fig. 2: S100A11 facilitates cell proliferation, migration and stem cell potential of PDAC cells.

From: S100A11 activates the pentose phosphate pathway to induce malignant biological behaviour of pancreatic ductal adenocarcinoma

Fig. 2

A, B S100A11 was knocked down using the CRISPR-Cas9 system. The knockdown efficiency was confirmed by western blotting (A) and quantative real-time PCR (B) in PDAC cells. C Control or S100A11-knockdown PDAC cells were plated on six-well plates and cell numbers were counted daily for 3 days. D, E S100A11 knockdown inhibited the proliferation of PDAC cells, as detected by colony formation assay. F, G Transwell assay indicated that S100A11 knockdown decreased the invasiveness of PDAC cells. H, I The spheroid formation assay revealed that knockdown of S100A11 impaired the ability of PDAC cells to form spheroids. J Representative tumours originating from control or S100A11-knockdown PDAC cells are shown. K CSC frequency in PDAC from an in vivo limiting dilution assay was calculated using ELDA software. *p < 0.05. Error bars indicate mean ± SD. Con, PDAC cells transfected with S100A11 control sequence; KD1, PDAC cells transfected with S100A11-specific knockdown sequence 1; KD2, PDAC cells transfected with S100A11-specific knockdown sequence 2.

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