Fig. 5: Detection and quantification of cytochrome c, calnexin, and ATF-4. | Cell Death & Disease

Fig. 5: Detection and quantification of cytochrome c, calnexin, and ATF-4.

From: Implementation of a combined CDK inhibition and arginine-deprivation approach to target arginine-auxotrophic glioblastoma multiforme cells

Fig. 5

A GBM cells (HROG63) were stained with respective antibodies to determine the subcellular localization of cytochrome c (green), calnexin (red), ATF-4 (violet) and counterstained with DAPI to visualize nuclei (blue). CDKi-SpyADI combination resulted in minor UPR. Merged images as well as single images were taken on a Leica DMI 4000B for mitochondrial function/acidic compartment evaluation and ER-stress marker and cytochrome c localization were analyzed via a Zeiss Elyra 7 Confocal Laser Microscope. Representative images are shown. B Quantification of specific markers. n = 3 independent experiments; *p < 0.05, **p < 0.01; ****p < 0.0001; §§§§p < 0.001; $$p < 0.01. One-way ANOVA.

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