Fig. 6: DNA-damage analysis using immunofluorescence and western blot. | Cell Death & Disease

Fig. 6: DNA-damage analysis using immunofluorescence and western blot.

From: Implementation of a combined CDK inhibition and arginine-deprivation approach to target arginine-auxotrophic glioblastoma multiforme cells

Fig. 6Fig. 6

A Representative images of 2D-cultured GBM cells (HROG05, HROG63), treated with test substances for 2 × 72 h or left untreated. Cells were stained with the following monoclonal antibodies: anti-H2A.X Phospho (Ser139) [red], anti-GADD45 [green], anti-β-catenin [red], and anti-Axin22. For the latter, Axin2, a secondary DyLight 488-conjugated anti-rabbit antibody was applied [green]. Scale bar as indicated: 50 µm. Nuclei were counterstained with DAPI. Images were taken on a Zeiss Elyra 7 Confocal Laser Microscope. B Quantification of specific markers. n = 3 independent experiments; ***p < 0.001; §§p < 0.01; $$p < 0.01. One-way ANOVA.

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