Fig. 5: Abnormal cilia structure and function in Dnah9 KD mice. | Cell Death & Disease

Fig. 5: Abnormal cilia structure and function in Dnah9 KD mice.

From: Dnah9 mutant mice and organoid models recapitulate the clinical features of patients with PCD and provide an excellent platform for drug screening

Fig. 5

A H&E staining of bronchi in lung sections in WT and Dnah9 KD mice at 40 days old. Compared to the WT, the tracheal epithelia of Dnah9 KD mice show vacuolization (blue arrows), and the distribution of cilia seems more clustered (black arrows). Boxed regions on the right depict higher magnifications. Scale bar = 10 μm. B The SEM showing surface of tracheal epithelia from WT and Dnah9 KD mice. Boxed regions are shown at higher magnification on the right. Scale bar = 9 μm. C TEM images of tracheal epithelium cilia for WT and Dnah9 KD mice. The axonemal structure is regular but shows a partially distal absence of ODAs in tracheal epithelium cilia of Dnah9 KD mice (red arrow). Scale bar = 200 nm. D Representative kymograph of 40-day-old WT and Dnah9 KD nasal cilia from high-speed video microscopy study. Scale bar = 0.005 s. E The CBF of Dnah9 KD mice is significantly reduced compared with WT mice. *P < 0.05. n = 6 per group.

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