Fig. 6: Ciliated cells from Dnah9 KD mice show reduced expression of interactors CCDC114 and GAS8 protein. | Cell Death & Disease

Fig. 6: Ciliated cells from Dnah9 KD mice show reduced expression of interactors CCDC114 and GAS8 protein.

From: Dnah9 mutant mice and organoid models recapitulate the clinical features of patients with PCD and provide an excellent platform for drug screening

Fig. 6

A Immunostaining analysis of DNAH9, CCDC114, LLRC6, and GAS8 in ciliated cells from Dnah9 KD nasal epithelium. Green, ace-tubulin; red, DNAH9, LRRC6, CCDC114, and GAS8; blue, DAPI; Scale bar = 10 µm. B Results of immunofluorescence images quantification corresponding to ciliated cells in (A). *P < 0.05; ns no significance, n = 3 per group. C Western blot analysis showing the protein expression of CCDC114, GAS8, and LRRC6 in lung tissues of WT and Dnah9 KD mice. GAPDH is the endogenous control. The right pattern is the statistical analysis of relative protein intensities revealed by western blot. *P < 0.05; ns no significance, n = 3 per group. D Co-IP results demonstrating interactions between DNAH9 and CCDC114/GAS8.

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