Fig. 7: Dnah9 KD mouse organoid shows reduced ciliary beating and increased secretion of inflammatory factors. | Cell Death & Disease

Fig. 7: Dnah9 KD mouse organoid shows reduced ciliary beating and increased secretion of inflammatory factors.

From: Dnah9 mutant mice and organoid models recapitulate the clinical features of patients with PCD and provide an excellent platform for drug screening

Fig. 7

A Bright-field images depicting mouse airway organoid phenotypes. Scale bar = 100 µm. B RT-PCR shows low DNAH9 expression in Dnah9 KD mouse airway organoid relative to WT. *P < 0.05; n = 3 per group. C CBF of Dnah9 KD group was significantly lower than WT group. *P < 0.05. n = 10 per group. D Whole-mount mouse airway organoid immunofluorescence shows markers for basal cells (KRT5, red), ciliated cells (acetylated a-tubulin, green), club cells (CC-10, green) and goblet cells (MUC5AC, green). Scale bar = 10 µm. E Confocal microscopy images of mouse airway organoid with a pH probe show lower pH value in the Dnah9 KD group than WT. The stronger red color indicates lower pH value in the epithelial cell of the Dnah9 KD group. Scale bar = 10 µm.

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