Fig. 4: SLC5A3 overexpression exerts pro-tumorigenic activity in NSCLC cells.

Patient-derived pCan-1 primary NSCLC cells, bearing SLC5A3-expressing lentiviral construct (oeSLC5A3-sL-1 and oeSLC5A3-sL-2, two different selections) were established, control cells were with the empty vector (“Vec”); SLC5A3 mRNA and protein expression was tested (A and B). After culturing for the designated hours, cell proliferation (C) and migration (D) were examined. The pCan-1 primary NSCLC cells stably expressing shSLC5A3-S1 were further transduced with the SLC5A3-expressing lentiviral construct (“+oeSLC5A3”) or the empty vector, expression of listed proteins was shown (E); After culturing for the designated hours, cell proliferation (F) and migration (F) were tested. The primary lung epithelial cells (“pEpi”), bearing the SLC5A3-expressing lentiviral construct (oeSLC5A3) or the empty vector (“Vec”), were established; SLC5A3 mRNA and protein expression was shown (G); After culturing for the designated hours, CCK-8 viability (H), cell proliferation (testing the percentage of EdU positively-stained nuclei, H), were examined. Data were presented as mean ± standard deviation (SD, n = 5). *P < 0.05 versus “Vec” group. *P < 0.05 (F). “N. S.” indicated no statistical difference (P > 0.05). Each single experiment was repeated for five times. Scale bar = 100 μm.