Fig. 6: SLC5A3 is important for Akt-mTOR activation in NSCLC cells.
From: The sodium/myo-inositol co-transporter SLC5A3 promotes non-small cell lung cancer cell growth
The stable pCan-1 primary cells, bearing the designated SLC5A3 lentiviral shRNA (shSLC5A3-S1 and shSLC5A3-S2), the lenti-CRISPR/Cas9-SLC5A3-KO construct (koSLC5A3), the scramble non-sense lentiviral shRNA plus the lenti-CRISPR/Cas9-KO empty vector (“shC+koC”), the SLC5A3-expressing lentiviral construct (oeSLC5A3-sL-1 and oeSLC5A3-sL-2) or the empty vector (“Vec”) were established; Expression of listed proteins was shown (A and B). The koSLC5A3 pCan-1 primary cells were further transduced with or without the adenoviral constitutively-active Akt1 (“caAkt1”, S473D), and stable cells established after selection. Expression of listed proteins was shown (C); Cells were further cultured for the designated time periods, and cell proliferation (by measuring EdU-positive nuclei ratio, D), in vitro cell migration (E) and apoptosis (by measuring TUNEL-positively stained nuclei ratio, F) were tested by the described methods. “Pare” indicated the parental control NSCLC cells. Data were presented as mean ± standard deviation (SD, n = 5). *P < 0.05 versus “Pare”/“Vec”/“koC”/“DMSO” group. #P < 0.05 versus “koSLC5A3” group (D–F). “N. S.” indicated no statistical difference (P > 0.05, A and B). Each single experiment was repeated for five times.
