Fig. 7: SLC5A3 is required for NSCLC xenograft growth in vivo.
From: The sodium/myo-inositol co-transporter SLC5A3 promotes non-small cell lung cancer cell growth
The pCan-1 xenograft-bearing nude mice were subject to intratumoral injection of the shSLC5A3-S1-expressing adeno-associated virus (AAV-shSLC5A3) or the scramble control shRNA adeno-associated virus (“AAV-shC”), daily for 10 days; The estimated tumor volumes (in mm3, A) and the mice body weights (in grams, C) were recorded every 5 days, from “Day-0” to “Day-35”. The mice were decapitated and killed at “Day-35”, and pCan-1 xenografts were weighted individually (B). At “Day-5” and “Day-10”, 4 h after AAV injection, one pCan-1 xenograft per group was isolated. Expression of listed genes and proteins in xenograft tumor lysates was measured (D, E, G and H); The myo-inositol (“MI”) contents were measured as well (F). Data were presented as mean ± standard deviation (SD). *P < 0.05 versus “AAV-shC” group. Scale bar = 100 μm (H).
