Fig. 1: IRAR is induced by ischemia reperfusion (IR) in mice. | Cell Death & Disease

Fig. 1: IRAR is induced by ischemia reperfusion (IR) in mice.

From: LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury

Fig. 1

A Screening strategy of IR-induced murine lncRNAs derived from microarray profiling. B A volcano plot showed the differentially expressed lncRNAs in IR group compared to Sham group. Red and green dots represent upregulated and downregulated lncRNAs in kidneys 24 h after IR, respectively (fold-change ≥ 2.0 and p-value ≤ 0.05). Black dots represent the genes that were not differentially expressed. IRAR is highlighted in red. C Time course of IRAR expression. Data represent mean ± SEM. n = 6-8, **p < 0.01 versus Sham group. D RNA fluorescence in situ hybridization (FISH) assay of IRAR in the mouse kidney samples at 24 h after IR. Scale bar, 100 μm. E FISH assay of IRAR in the hypoxia-treated mTECs. Scale bar, 25 μm. F The expression level of IRAR in cytoplasm and nuclei of hypoxia-treated mTECs. GAPDH (cytoplasm retained) and U1 (nuclear retained) were used as controls. G 3′ and 5′ rapid amplification of cDNA ends (RACE). Agarose gel electrophoresis of PCR products from the 5′-RACE and 3′-RACE procedure.

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