Fig. 5: IRAR regulates chemokine expression. | Cell Death & Disease

Fig. 5: IRAR regulates chemokine expression.

From: LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury

Fig. 5

A Co -expression network of IRAR and differentially expressed mRNAs. The solid line indicates positive regulation and the dotted line indicates negative regulation. B qRT-PCR analysis of IRAR in mTECs transfected with IRAR overexpressing lentivirus (LncRNA-OE) or Negative lentivirus (LncRNA-NC). C–E qRT-PCR analysis of CXCL1 (C), CXCL2 (D), CCL2 (E) in mTECs transfected with IRAR overexpressing lentivirus or Negative lentivirus. F Western blot analysis of CXCL1, CXCL2 and CCL2 in mTECs transfected with IRAR overexpressing lentivirus or Negative lentivirus.. Data are means from three independent experiments. G–J qRT-PCR analysis of IRAR (G), CXCL1 (H), CXCL2 (I) and CCL2 (J) in mTECs. mTECs were transfected with GapmeR IRAR (GapmeR-Hypoxia) or Negative control (Control-Hypoxia) for 48 h, then treated with hypoxia. K RNA fluorescence in situ hybridization (FISH) assays indicated co-expression between IRAR and CXCL1, CXCL2, CCL2 in the kidney at 24 h after ischemia reperfusion. Scale bar, 100 μm. L RNA immunoprecipitation (RIP) experiments were performed using antibodies against CXCL1, CXCL2 and CCL2. RIP enrichment was determined relative to the input controls. M RNA pulldown assays were performed in tubular epithelia cells to examine the association of IRAR with CXCL1, CXCL2 and CCL2. Data represent mean ± SEM. n = 4. **p < 0.01.

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