Fig. 7: Tubule-specific ablation of CCL2 reduces macrophages infiltration and kidndy injury. | Cell Death & Disease

Fig. 7: Tubule-specific ablation of CCL2 reduces macrophages infiltration and kidndy injury.

From: LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury

Fig. 7

A Breeding protocol for generating conditional tubular epithelial cell knockout mice (TE-CCL2-KO). B Representative gel images of genotyping. DNA was extracted from mouse tail and amplified, wild-type and floxed alleles of CCL2 and Cdh16-cre allele were detected. Lane 1 showed the genotyping of the wild-type mice used in this study (CCL2fl/fl), lane 2 showed the genotyping of the tubule-specific CCL2 knockout mice (CCL2fl/flCre, designated as TE-CCL2-KO). C Western blot analysis indicated a substantial reduction of renal CCL2 expression in TE-CCL2-KO mice 24 h after renal IR. D Representative images showed CCL2 staining in renal cortical and medullar regions of the WT and TE-CCL2-KO mice at 24 h after IR. Scale bar, 50 or 100 μm. E Immunofluorescence staining of F4/80 in the kidney 6 h after renal IR. Scale bar, 100 μm. F Quantification of F4/80-positive cells. G Concentration of serum creatinine 24 h after renal IR. H ELISA of IL-6 in the kidney 6 h after renal IR. Data represent mean ± SEM. n = 6–8. *p < 0.05, **p < 0.01.

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