Fig. 5: FMRP modulates the WNT canonical and non-canonical pathways.

A Left panel shows representative western blot of β-Catenin, GSK3β, and p-GSK3β levels in GSCs transduced with shNTC and shFMR1. Vinculin and Coomassie staining were used as normalisers. Right histogram represents the quantification of the biological replicates of p-GSK3β, β-Catenin and WNT5B (#148, #163, and #169 GSCs). p-GSK3β was normalised over total GSK3β levels (mean ± SEM, n = 3 independent cell lines, each data point averaged from three FACS sortings, **P < 0.01, ***P < 0.001, One-sample t-test). B Left panel shows representative western blot of ERK1/2 and p-ERK1/2 levels in GSCs transduced with shNTC and shFMR1. Vinculin and Coomassie staining were used as normalisers. Right histogram represents the quantification of the biological replicates (#148, #163, and #169 GSCs). p-ERK1/2 was normalised over total ERK1/2 levels (mean ± SEM, n = 3 independent cell lines, each data point averaged from three FACS sortings, ***P < 0.001, One-sample t-test). C Left panel shows representative Western blot of CREB, p-CREB, ETS1 and p-ETS1 levels in GSCs transduced with shNTC and shFMR1. Vinculin and Coomassie staining were used as normalisers. Right histogram represents the quantification of the biological replicates (#148, #163 and #169 GSCs). Phosphorylated proteins were normalised over total protein levels (mean ± SEM, n = 3 different cell lines, each data point is the average of three independent FACS sortings, *P < 0.05, **P < 0.01, One-sample t-test).