Fig. 3: IDO1 inhibitor suppressed DLBCL cell growth. | Cell Death & Disease

Fig. 3: IDO1 inhibitor suppressed DLBCL cell growth.

From: IDO1 plays a tumor-promoting role via MDM2-mediated suppression of the p53 pathway in diffuse large B-cell lymphoma

Fig. 3

A, B Immunofluorescence staining for IDO1 expression in PBMCs, OCI-Ly3 cells, and OCI-Ly10 cells (× 630, scale bar, 10 μm). C WB analysis of IDO1 protein expression in OCI-Ly3, OCI-Ly10, and PBMCs. D, E 1-L-MT competitively inhibited the activity of IDO1 and did not affect the protein expression level of IDO1 by immunofluorescence assay (× 630; scale bar, 10 μm). F, G Cell viability was determined using CCK-8 after OCI-Ly3 and OCI-Ly10 cells were treated with various concentrations of 1-L-MT for 48 h. H,I OCI-Ly3 and OCI-Ly10 cells were stimulated with 5 mM 1-L-MT or 50 μM INCB for 24 h, and then ELISA was used to determine the KYN acid secretion. J,K EdU staining of 5 mM 1-L-MT-treated and control OCI-Ly3/OCI-Ly10 cells (×100; scale bar, 100 μm). Statistical analysis was analyzed with a two-sided student’s t-test. *P < 0.05 and **P < 0.01.

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