Fig. 6: Ets-1 antagonism promotes sorafenib sensitivity in soraR cells. | Cell Death & Disease

Fig. 6: Ets-1 antagonism promotes sorafenib sensitivity in soraR cells.

From: Ets1 mediates sorafenib resistance by regulating mitochondrial ROS pathway in hepatocellular carcinoma

Fig. 6

A Hep3B-soraR cells transiently transfected with control-siRNA or Ets-1-siRNA were treated with DMSO or sorafenib (6 µM) for 48 h and analyzed by flow cytometry to detect apoptosis (A), mitochondrial damage (B), or caspase 3/7 activity (C). The bar graphs on the right of A–C represent the degree of apoptosis, mitochondrial damage, and caspase 3/7 activity respectively. Lanes 1 and 2 were transfected with control-siRNA and lanes 3 and 4 were transfected with Ets-1-siRNA and treated with DMSO (lanes 1, 3) or sorafenib (lanes 2, 4). The data represent the mean ± S.D. of at least three independent experiments. Significant differences were determined by t test and indicated as: *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. D Hep3B-soraR cells transfected with control- or Ets-1-siRNA were treated with DMSO or sorafenib (6 µM) for 48 h and analyzed by western blots. Cl PARP cleaved PARP.

Back to article page