Fig. 1: Ferroptosis exist in CVB3-induced myocardial injury in vivo.
From: TFRC upregulation promotes ferroptosis in CVB3 infection via nucleus recruitment of Sp1
A General basic morphology of the heart in the normal control group and the CVB3-infected group in Balb/c mice. The ratio of heat weight compared to body weight in two groups was shown in the statistical chart. n = 6. B The representative pictures of H&E staining in control group and CVB3 infection group mice myocardial (scale bar = 50 μm). C The chart represented the comparison of serum myocarditis inflammation indexes CK-MB and cTnI in control and CVB3 group mice. n = 6. D Transmission electron microscopy were used to detect the mitochondrial morphology and virus particles of myocardial tissue in control and CVB3 mice. The white arrow indicated mitochondria, and the black arrow indicated virus particles in CVB3 group (scale bar = 1 μm). E The level of CVB3 virus mRNA in myocardial was detected by qPCR. n = 6. F Iron concentrations were measured in the control and CVB3 groups using an Iron Assay Kit. n = 6. G MDA concentrations in the two groups myocardial were determined by Lipid Peroxidation MDA Assay Kit. n = 6. H GSH in myocardial tissue were detected using the GSH assay kit. n = 6. I, J Representative images of western-blotting and relative gray values on ferroptosis genes TFRC and ACSL4 protein expression. K, L Images of NCOA4 and Fth1 protein expression and relative gray values by western-blotting and Image J. n = 6. All results are expressed as the mean ± SD. t-test was used for comparison between two groups. *p < 0.05, **p < 0.01, ***p < 0.001.
