Fig. 2: Expression of HIF-1α and eIF5A2 were upregulated under hypoxic conditions.
From: Eukaryotic initiation factor 5A2 mediates hypoxia-induced autophagy and cisplatin resistance
A–D Western blotting (A–C) and qRT-PCR (D) results showing that the expression levels of HIF-1α and eIF5A2 in five NSCLC cell lines (A549, HCC827, NCI-H1703, PC9, and NCI-H1299) increased under hypoxic conditions. E A scatter plot suggesting that eIF5A2 levels correlated significantly and positively with ATG3 levels (R = 0.36, p < 0.001). F Luciferase activity analyzed after HEK293T cells were co-transfected with the indicated luciferase reporter plasmids, vector, or pcDNA eIF5A2. G qRT-PCR results suggesting that eIF5A2 expression in A549 and NCI-H1299 cells decreased after HIF-1α silencing. *P < 0.05, **P < 0.01.
