Table 2 Primers utilized for qRT-PCR in this study.
From: Eukaryotic initiation factor 5A2 mediates hypoxia-induced autophagy and cisplatin resistance
Gene | Primer sequence |
|---|---|
HIF-1α | forward 5’-ATCCATGTGACCATGAGGAAATG-3’ |
reverse 5’-TCGGCTAGTTAGGGTACACTTC-3’ | |
eIF5A2 | forward 5’-TGTCCTTCTACTCACAACATGGA-3’ |
reverse 5’-CTCACGAACTTCACCAGTTTCT-3’ | |
ATG3 | forward 5’-AACATGGCAATGGGCTAC-3’ |
reverse 5’-ATCTGTTTGCACCGCTTATAG-3’ | |
Beclin-1 | forward 5’-CTCCCGAGGTGAAGAGCATC-3’ |
reverse 5’-AATGGAGCTGTGAGTTCCTGG-3’ | |
p62 | forward 5’-GAAGCTGCCTTGTACCCACATC-3’ |
reverse 5’-TGTCATAGTTCTTGGTCTGCAGGAG-3’ | |
ACTB | forward 5’-TGGCACCCAGCACAATGAA-3’ |
reverse 5’-CTAAGTCATAGTCCGCCTAGAAGCA-3’ | |
GADPH | forward 5’-ATCATCAGCAATGCCTCC-3’ |
reverse 5’-TCCTTCCACGATACCAAAG-3’ |
