Fig. 4: METTL3 increased the abundance of m6A and enhanced the stability of lncRNA SNHG17. | Cell Death & Disease

Fig. 4: METTL3 increased the abundance of m6A and enhanced the stability of lncRNA SNHG17.

From: m6A methyltransferase METTL3-induced lncRNA SNHG17 promotes lung adenocarcinoma gefitinib resistance by epigenetically repressing LATS2 expression

Fig. 4

A m6AVar predicted potential m6A modification on the lncRNA SNHG17 sequence. B MeRIP-qPCR was performed to detect m6A expression in PC9 and A549 cells as well as in the corresponding gefitinib-resistant cells. C PC9, A549, PC9/GR and A549/GR cells were treated with actinomycin D for 0, 3 and 6 h, followed by the detection of lncRNA SNHG17 expression using qRT-PCR. D METTL3 expression in PC9, A549, PC9/GR and A549/GR cells. E qRT-PCR analysis of METTL3 mRNA level in PC9/GR and A549/GR cell lines after transfection with METTL3. F The m6A modification of PC9/GR and A549/GR cells transfected with METTL3 was examined by colorimetric quantificational analysis. G MeRIP-qPCR was performed to detect the m6A modification of lncRNA SNHG17 in METTL3-overexpressing PC9/GR and A549/GR cells. H RNA stability examination was performed to assess the influences of METTL3 overexpression on the stability of lncRNA SNHG17 in PC9/GR and A549/GR cells. Data were expressed as the mean ± SD of n = 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

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