Fig. 1: Activating mitotic SENP3 in tumor cells stimulates host anti-tumor immunity.
From: Mitotic SENP3 activation couples with cGAS signaling in tumor cells to stimulate anti-tumor immunity

A Colony formation assay in vitro. SENP3-WT-MC38 cells (WT) or SENP3–9A-MC38(9A) cells (500 cells/well) were cultured in six-well plate. Two weeks after seeding, the colonies containing more than 50 cells were recorded. Data are representative of three independent experiments and are shown as mean ± SD. **P < 0.01. B SENP3-WT-MC38 cells (WT) or SENP3–9A-MC38(9A) cells were subcutaneously injected into C57BL/6 mice (1 × 106/mouse, n = 5 mice). Tumor size was determined once in 3 days in two dimensions. Tumor growth curves represent the mean ± SD. Tumors were harvested and pictured on day 28 post-injection. Tumor weight was also measured immediately after harvest and the data from three independent experiments were shown in the bottom panel. Mean ± SD is indicated. **P < 0.01. C, D SENP3-WT-MC38 cells (WT) or SENP3–9A-MC38(9A) cells were subcutaneously injected into C57BL/6 mice (1 × 106/mouse, n = 5 mice). Tumors were harvested in 18 days’ post-injection and analyzed by flow cytometry (C). The cells were pre-gated on CD45+. Histogram shows the population of CD8+ T cells (C), NK1.1+, or CD4+ (D) in gated CD45+ cells. Data are representative of three independent experiments and are shown as mean ± SD. **P < 0.01. E SENP3-WT-MC38 cells (WT) or SENP3–9A-MC38(9A) cells were subcutaneously injected into C57BL/6 mice (1 × 106/mouse, n = 5 mice). Tumor draining inguinal lymph nodes (dLNs) were removed from mice in 18 days’ post-injection. CD8+ or CD4+ T cells in CD45+ cells were analyzed by flow cytometry. Data are representative of three independent experiments and are shown as mean ± SD. *P < 0.05. F SENP3-WT-MC38 cells (WT) or SENP3–9A-MC38(9A) cells were subcutaneously injected into C57BL/6 mice (1 × 106/mouse, n = 5 mice). On day 7 after injection, each kind of tumor-bearing mice was divided into two groups and anti-mouse CD8α antibodies were intraperitoneally injected (250 μg per mouse) on day 9, 13, and 17 after tumor injection. Tumor size was determined once in 3 days in two dimensions. Tumor growth curves represent the mean ± SD. Tumors were harvested on day 20 post-injection. Tumor weights were also measured immediately after harvest and the data were shown in histogram. Mean ± SD is indicated. **P < 0.01, *P < 0.05.