Fig. 3: Mitotic SENP3 activation promotes micronucleus formation. | Cell Death & Disease

Fig. 3: Mitotic SENP3 activation promotes micronucleus formation.

From: Mitotic SENP3 activation couples with cGAS signaling in tumor cells to stimulate anti-tumor immunity

Fig. 3

A SENP3-WT- or SENP3–9A-U2OS cells were stained with Hoechst. The micronuclei were analyzed by using a confocal microscope. The micronucleus staining positive cells (percentage of total cells) were quantitatively analyzed on 30 fields pictured by a confocal microscope. Data are represented as mean with SD. *P < 0.05. B SENP3 in U2OS cells were knocked out by using Cas9/CRISPER system plasmid px459 encoding human SENP3 gRNAs (Top of left panel). Then SENP3 KO U2OS cells were stably transfected with Doxycycline-induced SENP3-WT (WT) or SENP3 9A (9A) plasmids. The expression of SENP3-WT or SENP3–9A mutant in these cells were induced by the addition of doxycycline(1 μM/mL) for 48 h (bottom of left panel). The micronuclei were stained with Hoechst and quantitatively analyzed on 30 fields pictured by confocal microscope (right panel). Data are represented as mean with SD. **P < 0.01. C SENP3-WT- or SENP3–9A-U2OS cells were immune-stained with anti-Lamin B1 or anti-γH2AX antibody, and co-stained with DAPI. The co-localization of micronuclei with γH2AX was quantitatively analyzed on 30 fields pictured by confocal microscope. Data are represented as mean with SD. **P < 0.01.

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