Fig. 4: Mitotic SENP3 activation promotes cell senescence.

A MEF cells were stably transfected with mouse SENP3-WT or SENP3–9A plasmids. Then these cells were stained with SA-β-Gal (left panel). SA-β-Gal positive cells were quantitatively analyzed on 30 fields pictured by microscope (right panel). Data are represented as mean with SD. **P < 0.01. B p53-WT HCT116 cells were stably transfected with SENP3-WT or SENP3–9E plasmids and then treated with doxorubicin (1 μg/ml) for 1 h. Then these cells were stained with SA-β-Gal in 6 days after doxorubicin treatment. C SENP3-WT- or SENP3–9A-U2OS cells were treated with or without doxorubicin (1 μg/ml) for 1 h and harvested in 3 days after treatment. Real-time PCR was used for the analysis of CCL5 and IFNB1 expression in these cells. The data were normalized to GAPDH internal control and represented as mean with SD (n = 3 independent biological replicates). *P < 0.05. (ns) No significant. D p53-KO HCT116 cells were stably transfected with SENP3-WT or SENP3–9A plasmids. These cells were treated with or without doxorubicin (1 μg/ml) for 1 h and harvested 3 days after treatment. Real-time PCR was used for the analysis of CCL5 and IFNB1 expression in these cells. The data were normalized to GAPDH internal control and represented as mean with SD (n = 3 independent biological replicates). *P < 0.05. (ns) No significant.