Fig. 1: SARM1 was upregulated in the brain tissue of several ASD model mice.
From: SARM1 deletion in parvalbumin neurons is associated with autism-like behaviors in mice
A Western blot analysis of the expression levels of SARM1 in the cortex (Ctx), hippocampus (Hip), cerebellum (Cb), striatum (St), and thalamus (Th) of 2-month-old male mice. B Quantitative analysis of SARM1 expression (n = 6 animals per group). C, D Double immunostaining of SARM1 (green) and NeuN (red) (upper panel), SARM1 (green) and PV (red) (middle panel), SARM1 (green) and GFAP (red) (lower panel) in the cortex (C) or hippocampus (D) of 2-month-old male mice. Images of selected regions (white squares) are shown at a higher magnification. E Double immunostaining of SARM1 (red) and MAP2 (green) in primary cultured neurons in vitro. F Western blot analysis of the expression levels of SARM1 in the cortex (Ctx) and hippocampus (Hip) of 2-month-old control mice, Fmr1 KO mice, and LPS-induced autism-like mouse models. G Quantitative analysis of SARM1 expression as shown in (F) (n = 6 animals per group). The red asterisk represents the specific band of SARM1. The density of the western blot bands was normalized to that of the β-actin protein. Data are presented as the mean ± SEM. Quantitative data were analyzed using the Student’s t test and compared to the control group. ***p < 0.001. Scale bars, 20 μm.
