Fig. 4: Parvalbumin decreased in adult SARM1PV-CKO male mice.
From: SARM1 deletion in parvalbumin neurons is associated with autism-like behaviors in mice
A Immunostaining of PV (red) in the brain tissues of 2-month-old male SARM1f/f and SARM1PV-CKO mice. Cortex and hippocampus images are shown at a higher magnification. B Quantitative analysis of the number of PV+ cells in the cortex and hippocampus per mm2 as shown in (A) (n = 6 animals per group). C Western blot analysis of the expression of PV in the cortex and hippocampus of 2-month-old male SARM1f/f and SARM1PV-CKO mice. D Quantitative analysis of relative PV expression levels as shown in (C) (n = 6 animals per group). E qRT–PCR analysis of the mRNA expression levels of Pvalb in the cortex and hippocampus of 2-month-old male SARM1f/f and SARM1PV-CKO mice (n = 5 animals per group). β-actin was used as housekeeping gene. The density of the western blot bands was normalized to that of the β-actin protein. Quantitative data were analyzed using the Student’s t test and compared to the control group. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars, 1 mm.
