Fig. 3: Octyl-D-2-HG-treatment enhanced radiation-induced DNA damage and radiosensitized NCI-H460 cell lines. | Cell Death & Disease

Fig. 3: Octyl-D-2-HG-treatment enhanced radiation-induced DNA damage and radiosensitized NCI-H460 cell lines.

From: Accumulation of oncometabolite D-2-Hydroxyglutarate by SLC25A1 inhibition: A metabolic strategy for induction of HR-ness and radiosensitivity

Fig. 3

NCI-H460 cells were pre-treated for 2 h with solvent, CTPI2 (200 μM) or octyl-D-2-HG (150 μM) and subsequently exposed to irradiation with 0–30 Gy as indicated. DNA damage was quantified by alkaline comet assay a, c 24 h or b 6 h after the indicated treatments. d γ-H2AX signal was assessed by flow cytometry 6 h after the indicated treatment. e Colony formation assay was used to verify the survival fraction of NCI-H460 cells upon combined treatment as indicated. f Survival fraction (SF) of control (ctrl), CTPI2, or octyl-D-2-HG treatment groups in combination with IR (5 Gy) determined by colony formation assay. Data represent the mean values (±SD) from three independent experiments (N = 3). Statistical significance: one way ANOVA followed by Bonferroni post-test. ns = not significant (p > 0.05), *p < 0.05, ***p < 0.001, ****p < 0.0001.

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