Fig. 4: The CDN-binding domain of STING interacts with the CC domain of NCOA4. | Cell Death & Disease

Fig. 4: The CDN-binding domain of STING interacts with the CC domain of NCOA4.

From: The interaction between STING and NCOA4 exacerbates lethal sepsis by orchestrating ferroptosis and inflammatory responses in macrophages

Fig. 4

A Coimmunoprecipitation (Co-IP) of NCOA4 with STING in HEK293T cells. B Immunoprecipitation of anti-STING and anti-NCOA4 in PBMCs from healthy control and patients with sepsis. C Schematic structure of STING and NCOA4 protein. D Flag IP from lysates of HEK293T cells overexpressing Flag-tagged STING fragments and HA-tagged NCOA4 were immunoblotted. E Myc IP from lysates of HEK293T cells overexpressing Myc-tagged NCOA4 fragments and Flag-tagged STING1 were immunoblotted for detection of NCOA4. * Target bands. F Flag IP from lysates of HEK293T cells overexpressing Flag-tagged STING1 mutant plasmids and HA-tagged 1–245 NCOA4 fragments were immunoblotted. G Representative images of co-localization with STING (green) and 1–245 NCOA4 (red) fluorescent proteins were captured by laser scanning confocal microscopy. Enlarged images showed the confocal microscopy images of STING and NCOA4. All immunoblots are representative of at least three independent experiments with similar results. CBD CDN-binding domain, DD dimerization domain, CTT cytoplasmic-terminal tail, CC coiled-coil domain, FBD ferritin-binding domain, FL full-length, IP immunoprecipitation.

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