Fig. 1: Acidic pH decreases proliferation without effect on cell cycle in R54 GSCs.
From: Acidosis induces RIPK1-dependent death of glioblastoma stem cells via acid-sensing ion channel 1a
a Growth curves for R54 cells incubated in a medium with neutral (pH 7.4) or acidic pH (pH 6.6) with or without PcTx1. The cells were counted every 24 h for 4 days. Data are shown as mean ± SD, n = 6. b Doubling time of R54 cells, as determined with the data from panel (a). Data are shown as mean ± SD; *p < 0.05; **p < 0.01 (one-way ANOVA followed by Sidak’s post-hoc test). c Representative histograms of flow cytometry. d Percentage of cells in different phases of the cell cycle at pH 7.4 and at pH 6.6. e As in d but for pH 7.4 without and with PcTx1. f As in e, but for pH 6.6. The values for the DMSO condition in e, f are from panel (d). Data are shown as mean ± SD of nine samples from three independent experiments. Statistical analysis by two-way ANOVA with Bonferroni correction.
