Fig. 2: Acidic pH does not induce apoptosis in R54 GSCs.
From: Acidosis induces RIPK1-dependent death of glioblastoma stem cells via acid-sensing ion channel 1a
a Representative dot plots of flow cytometry measurements. b Percentage of viable, early apoptotic, and late apoptotic R54 cells at pH 7.4 and at pH 6.6. c As in b but for pH 7.4 with and without PcTx1. (d) as in c but for pH 6.6. The values for the DMSO condition in (c, d) are from panel (b). Data are shown as mean ± SD of nine samples from three independent experiments. Statistical analysis by two-way ANOVA with Bonferroni correction. e Normalized viability (mean ± SD) of R54 cells after 24 h incubation at pH 7.4 or at pH 6.6. n = 15 from three biological replicates. Statistical analysis by unpaired Student’s t-test.
