Fig. 3: Nec-1 rescues the reduced sphere formation rate at acidic pH.
From: Acidosis induces RIPK1-dependent death of glioblastoma stem cells via acid-sensing ion channel 1a
a, b Sphere numbers (mean ± SD) at pH 7.4 or at pH 6.6 at d12. 1 data point represents 1 experiment with n > 8 wells/experiment. c Representative images of spheroids after 12 days incubation at pH 7.4 or at pH 6.6. d, e SFR (mean ± SD) after 12 days incubation at pH 7.4 or at pH 6.6 with 20 µM Nec-1, 20 µM Z-Vad-FMK, or 10 µM IM-54. SFR was normalized to DMSO pH 7.4 control, which was 80.7 ± 24.5 for R54 and 62.2 ± 17.25 for R8. 1 data point represents 1 well. n ≥ 19 wells per condition from 2 (d, g, i) or 3 (e, f) independent experiments. (f, g) as in (d) but with 20 µM Nec-1, 20 µM Nec-1s or 2 µM Fer-1 with a mean of 47.4 ± 6.6 spheres for R54 and 72.4 ± 7.9 spheres for R8. h as in d but with 20 µM Nec-1s or 10 µM GSK’872 with a mean of 82.2 ± 8.9 spheres. i as in d, but with different concentrations of NSA, with a mean of 92 ± 12.8 spheres. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 (one-way ANOVA followed by Dunnett’s post-hoc test).
