Fig. 6: Transmission electron microscopy of R54 GSCs reveals necrosis at acidic pH early during sphere formation.
From: Acidosis induces RIPK1-dependent death of glioblastoma stem cells via acid-sensing ion channel 1a
a, b Representative TEM images of GSCs maintained for 1 day at pH 7.4 or at pH 6.6. White squares in a indicate regions that are shown on an expanded scale in (b). Arrows indicate plasma membrane, arrowheads mitochondria. c Representative TEM images of GSCs maintained for 7 days at pH 7.4 or at pH 6.6. d Pie charts indicating the percentage of cells with normal vs. necrotic morphology in TEM after 1 day or after 7 days at pH 7.4 or at pH 6.6. At d1, n = 106 for pH 7.4 and n = 118 for pH 6.6, respectively. At d7, n = 107 for pH 7.4 and n = 132 for pH 6.6, respectively. ****p < 0.0001 (Fisher’s exact test).
