Fig. 4: PHZ-OH attenuates bacterial sepsis in coordination of its antibacterial properties. | Cell Death & Disease

Fig. 4: PHZ-OH attenuates bacterial sepsis in coordination of its antibacterial properties.

From: Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling

Fig. 4

A Schematic illustration of the process of antibacterial activity examination; B Antibacterial effects of PHZ-OH at various doses against E. coli (106 CFU/ml for 6 h); Bacterial burdens of the plasma, the lung, the liver, and the spleen harvested 12 h after the challenge of CLP (C) or E. coli (D). Mice were treated with saline or 5 mg/kg of PHZ-OH 30 min prior to a challenge of CLP (ligating 75% of the cecal) or E. coli (ATCC 29522, 109 CFU per mice); E Survival curves of WT and Casp11−/− mice treated with saline or PHZ-OH (5 mg/kg) 30 min prior to a challenge of LPS (25 mg/kg); F Lung injury of WT and Casp11−/− mice treated with saline or PHZ-OH (5 mg/kg) 30 min prior to a challenge of LPS (25 mg/kg) or not for 12 h. Data are mean ± SEM of six mice in one experiment. *P < 0.05. ns, not significant. Scale bar = 50 μm.

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