Fig. 6: PHZ-OH inhibits LPS internalization and caspase-11-mediated TF activity. | Cell Death & Disease

Fig. 6: PHZ-OH inhibits LPS internalization and caspase-11-mediated TF activity.

From: Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling

Fig. 6

A Images of Proximity Ligation Assay (PLA) showing the accessing of LPS and caspase-11 (Red, Scar bar = 10 μm) within mouse peritoneal macrophages treated with saline or OMV (10 μg/ml) for 2 h in the presence or absence of PHZ-OH (4 μM); B Quantitative results of PLA. A spot means the average red points per cell in five random views of the indicated group within a slide; Cytotoxicity (C) and IL-1α/β release (D) of mouse peritoneal macrophages under a treatment of saline or PHZ-OH (4 μM) and/or a challenge of LPS (1 μg/ml) for 16 h with (E+) or without (E−) electroporation; Fluorescent staining of LPS (Red) (E) and LPS level of the cytosolic fraction assessed by using LAL assay (EU, endotoxin units) (F) in mouse peritoneal macrophages treated with saline or OMV (10 μg/mL) for 2 h in the presence or absence of PHZ-OH (4 μM); TF activity (G) and thrombin formation (H) of mouse peritoneal macrophages treated with saline or OMV (10 μg/mL) for 16 h in the presence or absence of PHZ-OH (4 μM). Data are shown as mean ± SEM of three independent experiments. *P < 0.05. ns, not significant.

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