Fig. 1: Apoptosis inhibits cytokine production.
From: Apoptotic caspase inhibits innate immune signaling by cleaving NF-κBs in both Mammals and Flies
A, B Quantitative RT-PCR analysis of CXCL10 and MCP1 mRNAs in 293 T cells or 293-TLR3 cells treated with Etop, TG, or ST in the absence or presence of IL-1β (10 ng/mL) for 4 hr (A), TNFα (10 ng/ml) for 2 h (B). C, D Quantitative RT-PCR analysis of Cxcl10 and Ifnb1 mRNA in macrophages from WT mice treated with Etop, TG and ST, and infected with HSV or SeV for 8 h. E Quantitative RT-PCR analysis of ifnb1, cxcl10 mRNA in peritoneal macrophages from WT mice treated with Etoposide (Etop, 100 μM, 24 h), Thapsigargin (TG, 2 μM, 24 h) and Staurosporine (ST, 1 μM, 8 h), then treated with poly(I:C) (2 μg/ml, 2 h). F, G Sex- and age-matched mice (n = 10) were ip injected of 20 mg/kg doxorubicin (DOX or Doxo) or PBS for 5 days, ip injected with poly(I:C) or PBS for 2 h. Cxcl10 and il-6 mRNAs of heart were analyzed by qRT-PCR (F), serum concentrations of the indicated cytokines were measured by ELISA (G). Data are representative of three independent experiments (means with SEMs). *p < 0.05, **p < 0.01, and ***p < 0.001.
