Fig. 2: Caspase-3 downregulates cytokine production.
From: Apoptotic caspase inhibits innate immune signaling by cleaving NF-κBs in both Mammals and Flies
A Quantitative RT-PCR analysis of CXCL10, IKBA and MCP1 mRNA in 293 T cells transfected with CASP3-specific siRNA (siCASP3) and treated with IL-1β (10 ng/mL) for 4 h in the presence or absence of Etop and TG as indicated. B Quantitative RT-PCR analysis of CXCL10 and IFNB1 mRNA in 293-TLR3 and CASP3-KO 293-TLR3 cells treated with or without poly(I:C). C Quantitative RT-PCR analysis of Cxcl10 and Ifnb1 mRNA in primary mouse peritoneal macrophages transfected with control siRNA or indicated siRNA in the absence or presence of poly(I:C). D, E Quantitative RT-PCR analysis of Cxcl10 and Ifnb1 mRNA in primary mouse peritoneal macrophages treated with or without z-VAD-FMK and infected with HSV (D) or SeV (E) for 8 h. F CASP3+/+ and CASP3−/− mice were injected with poly(I:C) for 2 h. Serum concentrations of the indicated cytokines were measured by ELISA. Data are representative of three independent experiments (means with SEMs). *p < 0.05, **p < 0.01, and ***p < 0.001.
