Fig. 3: Caspase-3 downregulates cytokine production induced by p65/RelA.
From: Apoptotic caspase inhibits innate immune signaling by cleaving NF-κBs in both Mammals and Flies
A, B Luciferase assay analyzing NF-κB and ISRE promoter activity in 293-TRL3 cells transfected for 48 h with the plasmids encoding NF-κB or ISRE firefly luciferase reporters and TK Renilla luciferase reporter for control, along with transfected the plasmid or treated with poly(I:C) (A) and Etop, TG, ST (B) as indicated. C qRT-PCR analysis of CXCL10, IL1B and TNFA mRNA in HeLa cells transfected with the Flag-p65 plasmid and treated with Etop or TG as indicated. Data are representative of three independent experiments (means with SEMs). *p < 0.05, **p < 0.01, and ***p < 0.001.
