Fig. 1: β-Ala prevents the OGD-induced CGC death.
From: Multi-target action of β-alanine protects cerebellar tissue from ischemic damage
A An experimental arrangement for monitoring ischemic changes in neuronal viability vs. cell death in the CGC layer, using two-photon excitation (2PE) imaging in acute cerebellar slices. B Representative images of Z-projection in the CGC layer to show FDA labeling of the cells (green fluorescence channel, indicates viable cells), PI staining (red fluorescence channel, indicates dead cells) and merged image. Images were taken as 512 × 512 frame scans over tissue depth: total scanned 48-μm depth; λ2Pex = 820 nm. C Examples of time-lapse 2PE images (merged FDA and PI) taken from the same region of interest in the CGC layer over the time of recording in different experimental conditions. From top to bottom: images of the CGC layer (Z-projection) in a control acute cerebellar tissue, a slice subjected to OGD (30-m duration) after 30-m control time interval followed by reperfusion, and tissue subjected to OGD in the presence of β-Ala, as denoted. Time marks at the bottom apply to all images; the “0 m” time mark denotes the start point of OGD (30-m duration). D Summary of image analyses for the proportion of viable (FDA fluorescence) vs. dead CGCs (PI, red fluorescence) over the time in control (n = 6 slices/4 animals, left plots), OGD (n = 8 slices/4 animals, middle) and OGD in the presence of β-Ala (n = 5 slices/3 animals, right plots). Data normalized to the control values (image captured 30 m before the OGD onset corresponds to 100% level). Notes apply to all three plots.
