Fig. 3: Single-channel recordings demonstrate the effect of β-Ala on GABAaRs, NMDARs, and GlyRs, with the concentration-dependent effects of the selective receptor ligands.
From: Multi-target action of β-alanine protects cerebellar tissue from ischemic damage
A Top: recordings of the single-channel GABAaR openings elicited by 1 mM of β-Ala and different concentrations of a selective GABAaR agonist muscimol (MSC) in the same outside-out patch (n = 8 recordings/6 animals). Bottom: statistical summary of the GABAaR open-time fraction, normalized to the response produced by 1 mM β-Ala. MSC at the concentration of 1 µM produces the equipotent effect as that elicited by β-Ala. B Same as in A, but for the single-channel NMDAR openings activated by 1 mM of β-Ala and different concentrations of Felbamate (Flb). 1 mM Flb was found as the equipotent concentration to β-Ala (n = 10 patches/cells per paired recordings made from 7 animals). C Same as in A, but for the single-channel GlyR openings activated by β-Ala and different concentrations of hypotaurine (HPT). HPT at the concentrations of 0.5 mM and 1 mM produced the equipotent effect to that of β-Ala (n = 8 recordings/8 animals). Scale bars and vertical axis label apply to A–C. *P < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant compared to the effect of β-Ala (Student’s paired t-test).
