Fig. 6: Overexpression of lncRNA HEPFAL decreased the stability of SLC7A11 protein.

A SLC7A11 immunofluorescence in HCCLM3 and PLC cells transfected as indicated. B Western blot analysis of SLC7A11 in HCCLM3 cells after transfected with lncRNA HEPFAL expression vector or control vector and treated with 20 μM MG-132 or untreated for 8 h. C, D LncRNA HEPFAL expression vector or control vector was transfected into HCCLM3 cells. After the cells were treated with or without 20 μM MG-132 for 8 h, SLC7A11 was immunoprecipitated with anti-SLC7A11 antibody, and the ubiquitination of SLC7A11 was examined by western blot. E, F HCCLM3 cells transfected with lncRNA HEPFAL expression vector or control vector were treated with 50 mg/ml CHX and detected the expression of SLC7A11 by western blot. The intensity of endogenous SLC7A11 expression for each time point was quantified by densitometry and drawn into a line chart.